Isotopically tagged synons from 2 carbon precursors

ABSTRACT

The use of vinyl sulfides, sulfoxides and sulfones in synthetic chemistry for the production of a wide variety of materials is well known. For example, phenyl vinyl sulfides, sulfoxides and sulfones have been used for the synthesis of important heterocycles, in combinatorial chemistry and as Diels-Alder adducts. Although these compounds have been used extensively for a variety of applications, the isotopically labeled versions have not been reported. A simple route for the isotopically labeled production of these important building blocks has been developed.

This patent application claims the priority and benefit of two U.S.Provisional Patent Applications the first being No. 60/923,107 filedApr. 11, 2007 entitled “Carbon Labeled, Isotopically Labeled C13Molecules and also deuterium Labeled Molecules Based on the Chemistry ofC13 Methyl Phenyl Sulfide” and the second being No. 60/948,359 filedJul. 6, 2007 and titled “Synthesis of Isotopically Tagged Synons”. Both60/923,107 and 60/948,359 are herein incorporated by reference.

TECHNICAL FIELD

The present invention relates to labeled compounds and more particularlyto isotopically enriched alkyl and aryl vinyl sulfides, sulfoxides andsulfones, labeled with isotopes of carbon and helium. The isotopic tagscan be carbon-13 or carbon-13 and hydrogen-2. The specific labeledcompounds are produced from two carbon precursors.

BACKGROUND OF THE INVENTION

Vinyl sulfides, sulfoxides and sulfones are extremely useful for thesynthesis of many important biochemical's and pharmaceuticals.Additionally, the use of stable isotopes has long been considered to bea promising tool in biomedical diagnosis. Furthermore, the past twodecades have seen a tremendous leap forward in the development of verysophisticated instrumentation for the detection of disease and forprobing biological structure and function. In conjunction with this aneed for very complicated isotopically labeled materials has been on theincrease.

Another area of application has become critical after the “9/11”tragedies. The use of stable isotopes in molecules (metabolites) for therapid detection of threat agents (chemical and biological) is now inlarge demand. Current isotopic labeling precursors and techniques,however, have made this a very daunting task. Some advancements havebeen previously disclosed in U.S. Pat. No. 6,753,446, U.S. Pat. No.6,709,645, U.S. Pat. No. 6,541,671, U.S. 2003158445, U.S. 2003153789,and U.S. 20030114. In order to meet the urgent and growing demand,further high purity isotopically labeled compounds are needed.

BRIEF SUMMARY

The following summary is provided to facilitate an understanding of someof the innovative features unique to the embodiments and is not intendedto be a full description. A full appreciation of the various aspects ofthe embodiments can be gained by taking the entire specification,claims, drawings, and abstract as a whole.

It is therefore an aspect of the embodiments to use known precursorssuch as [¹³C]Methyl phenyl sulfide to produce labeled two carbonprecursors such as [¹³C₂]Ethyl phenyl sulfide. The labeled two carbonprecursors can then be used to produce further previously unknownlabeled compounds. High purity precursors ensure that the new compoundsare also highly pure. For example, [¹³C]Methyl phenyl sulfide inconcentrations over 98 percent can be obtained using currently knowntechniques. As such, the previously unknown compounds herein disclosedare nearly 100 percent pure and are certainly over 90 percent pure.

BRIEF DESCRIPTION OF THE DRAWINGS

The accompanying figures, in which like reference numerals refer toidentical or functionally similar elements throughout the separate viewsand which are incorporated in and form a part of the specification,further illustrate aspects of the embodiments and, together with thebackground, brief summary, and detailed description serve to explain theprinciples of the embodiments.

FIG. 1 illustrates synthesis and reactions of [1,2-¹³C₂]ethyl phenylsulfide in accordance with aspects of the embodiments;

FIG. 2 illustrates synthesis of 2-phenylthio[1,2-¹³C₂]ethanol inaccordance with aspects of the embodiments;

FIG. 3 illustrates reactions of 2-phenylthio[1,2-¹³C₂]ethanol inaccordance with aspects of the embodiments;

FIG. 4 illustrates synthesis of vinyl sulfoxides in accordance withaspects of the embodiments;

FIG. 5 illustrates an alternative preparation of vinyl sulfones inaccordance with aspects of the embodiments;

FIG. 6 illustrates the synthesis of isotopically labeled ethers and analternative synthesis of vinyl sulfoxides and in particular thesynthesis of benzyl[1,2-¹³C₂]vinyl ether and phenyl[1,2-¹³C₂]vinylsulfoxide in accordance with aspects of the embodiments;

FIG. 7 illustrates a general formula for certain labeled compounds inaccordance with aspects of the embodiments;

FIG. 8 illustrates another general formula for additional labeledcompounds in accordance with aspects of the embodiments; and

FIG. 9 illustrates yet another general formula for yet additionallabeled compounds in accordance with aspects of the embodiments.

DETAILED DESCRIPTION

The following description contains a series of examples whereinpreviously known labeled compounds are processed to yield highly purelabeled compounds that are not previously known.

Synthesis of [1,2-¹³C₂]ethyl phenyl sulfide

FIG. 1 illustrates synthesis and reactions of [1,2-¹³C₂]ethyl phenylsulfide in accordance with aspects of the embodiments. A sample of[¹³C]methyl phenyl sulfide (15.0 g, 0.119 moles, 1 equivalent) wasdissolved in tetrahydrofuran (150 mL) in a 1 L round bottom flask. Thereaction vessel was then cooled to −78° C. using a dry-ice/ethanol bath.To this cooled reaction sec-butyl lithium (1.4M/THF, 94.24 mL, 0.1319moles, 1.3 equivalents) was added slowly over a 45-minute period. Thereaction was allowed to stir for 30 minutes and then [¹³C]methyl iodide(17.14 g, 0.1199 moles, 1 equivalent) was added slowly over a 40-minuteperiod to the reaction. The reaction was allowed to stir for anadditional hour and allowed to come to ambient temperature. After thisperiod, water (100 mL) was added to the reaction. The reaction was thenevaporated to remove tetrahydrofuran. To this mixture dichloromethane(150 mL) was added and the layers were separated. The organic layer waswashed with water (2×20 mL) and then dried with sodium sulfate and thenconcentrated in vacuo to give the pure product (16.1 g, 91%). Theproduct was suitable for use in subsequent reactions withoutpurification.

2-(Phenylthio)[U-¹³C₂]ethanol

FIG. 2 illustrates synthesis of 2-phenylthio[1,2-¹³C₂]ethanol inaccordance with aspects of the embodiments. Ethyl (phenylthio)[U-¹³C₂]acetate (5.0 g, 25.22 mmol, 1.0 eq.) was dissolved in THF (50mL) in a 250 mL round bottom flask, equipped with a magnetic stirrer,flushed with argon, and was cooled using and ice-water bath. Lithiumborohydride (2.0M, 25.2 mL, 2.0 equivalents) was added dropwise over aperiod of seven minutes. The reaction mixture was permitted to warm toroom temperature slowly as the ice melted, while stirring under argon.The reaction progress was monitored by TLC (at 1, 2, 8 and 24 hours) and¹³C NMR (2, 8 and 24 hours) by taking an aliquot from the reactionmixture, dissolving in CDCl₃, and monitoring the disappearance of ethyl(phenylthio) [U-¹³C₂]acetate and the subsequent appearance of thedesired 2-(phenylthio)[U-¹³C₂]ethanol. The reaction was complete after24 hours. The reaction mixture was cooled using an ice-water bath thenneutralized using 1 N HCl. The expected product was isolated byextraction with dichloromethane (3×50 mL). The organic layer wasevaporated by vacuum distillation using a rotary evaporator to yield apale yellow liquid (3.81 g, 97%), which was used without furtherpurification.

FIG. 3 illustrates reactions of 2-phenylthio[1,2-¹³C₂]ethanol inaccordance with aspects of the embodiments.2-phenylthio[1,2-¹³C₂]ethanol can be used as a precursor in theproduction of a number of isotopically tagged compounds.

2-Chloro[U-¹³C₂]ethyl phenyl sulfone

2-(Phenylthio) [U-¹³C₂]ethanol (1.0 g, 6.4 mmol, 1.0 equivalent) wasdissolved in dichloromethane (10 mL) in a 50 mL round bottom flask,equipped with a magnetic stirrer. 1.09 grams of silica was placed into1.0 mL of distilled water and then added to the stirred solution. Themixture was cooled using an ice-water bath. Once cooled, sulfurylchloride (1.6 mL, 3.0 equivalents) was added dropwise over a period ofapproximately 3 minutes. The reaction mixture was permitted to warm toroom temperature slowly as the ice melted. The reaction progress wasmonitored by ¹³C NMR (15 min.) by taking an aliquot from the reactionmixture, dissolving in CDCl₃, and monitoring the disappearance of2-(phenylthio) [U-¹³C]ethanol and the subsequent appearance of thedesired 2-chloro[U-¹³C]ethyl phenyl sulfone and was found to have goneto completion. The reaction mixture was cooled using an ice-water baththen neutralized using a saturated solution of sodium bicarbonate untilit reached a pH of 8-9. The mixture was then extracted usingdichloromethane (3×50 mL). The volatiles were removed by vacuum using arotary evaporator to yield a white solid (1.1 g, 97%), which was usedwithout further purification.

Phenyl[U-¹³C₂]vinyl sulfone

2-Chloro[U-¹³C₂]ethyl phenyl sulfone (1.1 g, 5.57 mmol, 1.0 eq.) wasdissolved in THF (12.5 mL) in a 50 mL round bottom flask, equipped witha magnetic stirrer. The mixture was warmed to approximately 30° C. usinga water bath. Once the solution warmed, triethylamine (1.2 mL, 1.5 eq.)dissolved in THF (10 mL) was added dropwise over a period of a minute.Salt formation was observed instantly. The reaction mixture waspermitted to cool to room temperature. The reaction progress wasmonitored by ¹³C NMR (17 hrs.) by taking an aliquot from the reactionmixture, dissolving in CDCl₃, and monitoring the disappearance of2-chloro[U-¹³C₂]ethyl phenyl sulfone and the subsequent appearance ofthe desired phenyl[U-¹³C₂]vinyl sulfone and was found to have gone tocompletion. The reaction mixture was filtered to remove the salt andwashed with additional THF. The solid that formed was purified usingcolumn chromatography to yield a white solid (0.85 g, 88%).

FIG. 4 illustrates synthesis of vinyl sulfoxides in accordance withaspects of the embodiments. Ethyl 2-(phenylthio)[1,2-¹³C₂]acetate can beused to produce 2-(phenylsulfinyl)[1,2-¹³C₂, 2,2-d₂]ethanol which canthen in turn be used to produce phenyl[1,2-¹³C₂,2,2-d₂]vinyl sulfoxide.The synthetic route can be used to produce all of the isotopiccombinations of the vinyl sulfides, sulfoxides and sulfoxides.

FIG. 5 illustrates an alternative preparation of vinyl sulfones inaccordance with aspects of the embodiments. Ethyl2-(phenylthio)[1,2-¹³C₂]acetate can be used to produce2-(phenylsulfinyl)[1,2-¹³C₂,2,2-d₂]chloride which can then in turn beused to produce phenyl[1,2-¹³C₂,2,2-d₂]vinyl sulfoxide.

FIG. 6 illustrates the synthesis of isotopically labeled ethers and analternative synthesis of vinyl sulfoxides and in particular thesynthesis of benzyl[1,2-¹³C₂]vinyl ether and phenyl[1,2-¹³C₂]vinylsulfoxide in accordance with aspects of the embodiments. A series ofreactions beginning with ethyl (benzyloxy) [1,2-¹³C₂]acetate produce anumber of isotopically tagged intermediary compounds and culminate inthe production of benzyl[1,2-¹³C₂]vinyl ether and phenyl[1,2-¹³C₂]vinylsulfoxide.

FIGS. 7-9 illustrates general formulas for certain labeled compounds inaccordance with aspects of the embodiments. The following examplesillustrate techniques for the synthesis of compounds described by thosegeneral formulas.

2-(Phenylthio) [U-¹³C₂]ethanol

Ethyl (phenylthio) [U-¹³C₂]acetate (5.0 g, 25.22 mmol, 1.0 eq.) wasdissolved in THF (50 mL) in a 250 mL round bottom flask, equipped with amagnetic stirrer, flushed with argon, and was cooled using and ice-waterbath. Lithium borohydride (2.0M, 25.2 mL, 2.0 equivalents) was addeddropwise over a period of seven minutes. The reaction mixture waspermitted to warm to room temperature slowly as the ice melted, whilestirring under argon. The reaction progress was monitored by TLC (at 1,2, 8 and 24 hours) and ¹³C NMR (2, 8 and 24 hours) by taking an aliquotfrom the reaction mixture, dissolving in CDCl₃, and monitoring thedisappearance of ethyl (phenylthio) [U-¹³C₂]acetate and the subsequentappearance of the desired 2-(phenylthio)[U-¹³C₂]ethanol. The reactionwas complete after 24 hours. The reaction mixture was cooled using anice-water bath then neutralized using 1 N HCl. The expected product wasisolated by extraction with dichloromethane (3×50 mL). The organic layerwas evaporated by vacuum distillation using a rotary evaporator to yielda pale yellow liquid (3.81 g, 97%), which was used without furtherpurification.

2-Chloro[U-¹³C₂]ethyl phenyl sulfone

2-(Phenylthio) [U-¹³C₂]ethanol (1.0 g, 6.4 mmol, 1.0 equivalent) wasdissolved in dichloromethane (10 mL) in a 50 mL round bottom flask,equipped with a magnetic stirrer. 1.09 grams of silica was placed into1.0 mL of distilled water and then added to the stirred solution. Themixture was cooled using an ice-water bath. Once cooled, sulfurylchloride (1.6 mL, 3.0 equivalents) was added dropwise over a period ofapproximately 3 minutes. The reaction mixture was permitted to warm toroom temperature slowly as the ice melted. The reaction progress wasmonitored by ¹³C NMR (15 min.) by taking an aliquot from the reactionmixture, dissolving in CDCl₃, and monitoring the disappearance of2-(phenylthio) [U-¹³C]ethanol and the subsequent appearance of thedesired 2-chloro[U-¹³C]ethyl phenyl sulfone and was found to have goneto completion. The reaction mixture was cooled using an ice-water baththen neutralized using a saturated solution of sodium bicarbonate untilit reached a pH of 8-9. The mixture was then extracted usingdichloromethane (3×50 mL). The volatiles were removed by vacuum using arotary evaporator to yield a white solid (1.1 g, 97%), which was usedwithout further purification.

Phenyl[U-¹³C₂]vinyl sulfone

2-Chloro[U-¹³C₂]ethyl phenyl sulfone (1.1 g, 5.57 mmol, 1.0 eq.) wasdissolved in THF (12.5 mL) in a 50 mL round bottom flask, equipped witha magnetic stirrer. The mixture was warmed to approximately 30° C. usinga water bath. Once the solution warmed, triethylamine (1.2 mL, 1.5 eq.)dissolved in THF (10 mL) was added dropwise over a period of a minute.Salt formation was observed instantly. The reaction mixture waspermitted to cool to room temperature. The reaction progress wasmonitored by ¹³C NMR (17 hrs.) by taking an aliquot from the reactionmixture, dissolving in CDCl₃, and monitoring the disappearance of2-chloro[U-¹³C₂]ethyl phenyl sulfone and the subsequent appearance ofthe desired phenyl[U-¹³C₂]vinyl sulfone and was found to have gone tocompletion. The reaction mixture was filtered to remove the salt andwashed with additional THF. The solid that formed was purified usingcolumn chromatography to yield a white solid (0.85 g, 88%).

The embodiments of the invention in which an exclusive property or rightis claimed are defined as follows. Having thus described the inventionwhat is claimed is:

1. A labeled compound having the structure:

wherein n 12 or 13, and m=12 or 13 with the proviso that both n and m donot simultaneously equal 12; wherein X═S, S0, S0₂, or O; whereinR¹=Alkyl or Aryl; wherein R²═H, ²H, ³H, Alkyl, or Aryl; wherein R³═H,²H, ³H, Alkyl, or Aryl; wherein R⁴═H, ²H, ³H, Alkyl, or Aryl; and withthe provisos that X≠O when m=13, n=12 and R¹ is methyl, and that X≠Owhen m=12, n=13 and R¹ is methyl.
 2. The labeled compound of claim 1wherein n=13, m=13, X═S0, R¹=Phenyl; R²═H, R³═²H, and R⁴═²H.
 3. Thelabeled compound of claim 1 wherein n=13, m=13, X═S0, R¹=Aryl; R²═H,R³═²H, and R⁴═²H.
 4. The labeled compound of claim 1 wherein n=13, m=13,X═S0₂, R¹=Phenyl; R²═H, R³═H, and R⁴═H.
 5. The labeled compound of claim1 wherein n=13, m=13, X=0, R¹=Phenyl; R²═H, R³═H, and R⁴═H.
 6. Thelabeled compound of claim 1 wherein n=13, m=13, X═S0, R¹=Phenyl; R²═H,R³═H, and R⁴═H.
 7. The labeled compound of claim 1 wherein n=13, m=13,X═S, R¹=Phenyl; R²═H, R³═²H, and R⁴═²H.
 8. A labeled compound having thestructure:

wherein n=12 or 13 and m=12 or 13 with the proviso that both n and m donot simultaneously equal 12; wherein X¹═S, S0, S0₂, or O; wherein X²═S,S0, S0₂, or O; wherein R¹=Alkyl or Aryl; wherein R⁶=Alkyl or Aryl;wherein R²═H. ²H, ³H, Alkyl, or Aryl; wherein R³═H, ²H, ³H, Alkyl, orAryl; wherein R⁴═H, ²H, ³H, Alkyl, or Aryl; wherein R⁵═H. ²H, ³H, Alkyl,or Aryl.
 9. The labeled compound of claim 8 wherein n=13, m=13, X¹═S0,X²═O; wherein R¹=Phenyl; R⁶=Ac, R²═H, R³═H, R⁴═²H, and R⁵═²H.
 10. Thelabeled compound of claim 8 wherein n=13, m=13, X¹═S0, X²═O; whereinR¹=Aryl; R⁶=Ac, R²═H, R³═H, R⁴═²H, and R⁵═²H.
 11. The labeled compoundof claim 8 wherein n=13, m=13, X¹═S0, X²═O; wherein R¹=Phenyl;R⁶=Phenyl, R²═H, R³═H, R⁴═H, and R⁵═H.
 12. A labeled compound having thestructure:

wherein n=12 or 13 and m=12 or 13 with the proviso that both n and m donot simultaneously equal 12; wherein X¹═S, S0, S0₂, or O; wherein X²═H,OH, or Cl; wherein R¹=Alkyl or Aryl; wherein R²═H, ²H, ³H, Alkyl, orAryl; wherein R³═H, ²H, ³H, Alkyl, or Aryl; wherein R⁴═H, ²H, ³H, Alkyl,or Aryl; wherein R⁵═H, ²H, ³H, Alkyl, or Aryl; with the proviso thatR¹≠Phenyl, Methyl, or Ethyl when n=12, m=13, X¹═S, X²=OH, R²═H; R³═H,R⁴═H, and R⁵═H; and with the further proviso that R¹≠Ethyl when n=13,m=12, X¹═S, X²═OH, R²═H; R³═H, R⁴═H, and R⁵═H.
 13. The labeled compoundof claim 12 wherein X²═OH.
 14. The labeled compound of claim 12 whereinn=13, m=13, X¹═O; X²═OH; R¹=Phenyl, R²═H, R³═H, R⁴═H, and R⁵═H.
 15. Thelabeled compound of claim 12 wherein n=13, m=13, X¹═S0₂; X²═Cl;R¹=Phenyl, R²═H, R³═H, R⁴═H, and R⁵═H.
 16. The labeled compound of claim12 wherein n=13, m=13, X¹═S; X²═H; R¹=Phenyl, R²═H, R³═H, R⁴═H, andR⁵═H.
 17. The labeled compound of claim 12 wherein n=13, m=13, X¹═S0;X²═H; R¹=Phenyl, R²H, R³═H, R⁴═H, and R⁵═H.
 18. The labeled compound ofclaim 12 wherein n=13, m=13, X¹═S0₂; X²═H; R¹=Phenyl, R²═H, R³═H, R⁴═H,and R⁵═H.
 19. The labeled compound of claim 12 wherein n=13, m=13, X¹═S;X²═OH; R¹=Phenyl, R²═H, R³═H, R⁴═H, and R⁵═H.
 20. The labeled compoundof claim 12 wherein n=13, m=13, X¹═S0₂; X²═OH; R¹=Phenyl, R²═H, R³═H,R⁴═H, and R⁵═H.
 21. The labeled compound of claim 12 wherein n=13, m=13,X¹═S; X²═OBn; R¹=Phenyl, R²═H, R³═H, R⁴═H, and R⁵═H.